pET28a_T7-ARG1-LUX vector (V017623)

LOCUS       .                      13790 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      12..467
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(562..1374)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1496..2084
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2270..2409)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2514..2702)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(3477..3498)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3514..4593)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(4594..4671)
                     /label="lacI promoter"
     promoter        4980..4998
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4999..5023
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     CDS             5125..5337
                     /gene="gvpA"
                     /label="gvpA"
                     /note="Gas vesicle protein A from Dolichospermum flosaquae.
                     Accession#: P10397"
     CDS             5446..5658
                     /gene="gvpA"
                     /label="gvpA"
                     /note="Gas vesicle protein A from Dolichospermum flosaquae.
                     Accession#: P10397"
     CDS             5894..6472
                     /gene="gvpC"
                     /label="gvpC"
                     /note="Gas vesicle protein C from Dolichospermum flosaquae.
                     Accession#: P09413"
     CDS             6506..8155
                     /label="luciferase"
                     /note="firefly luciferase"
     terminator      13718..13765
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"