Basic Vector Information
- Vector Name:
- mCherry-Rab5a-7
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5331 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- CMV
- 5' Primer:
- MCHERRY-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
mCherry-Rab5a-7 vector Map
mCherry-Rab5a-7 vector Sequence
LOCUS . 5331 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
enhancer 16..319
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 320..523
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 568..1275
/label="mCherry"
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
CDS 1297..1941
/gene="RAB5A"
/label="RAB5A"
/note="Ras-related protein Rab-5A from Homo sapiens.
Accession#: P20339"
polyA_signal 2074..2195
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2202..2657)
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2684..2788
/label="AmpR promoter"
promoter 2790..3147
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 3182..3973
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase from Tn5"
polyA_signal 4208..4255
/label="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4584..5172
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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