PPRE-H2B-eGFP vector (V017591)

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Basic Vector Information

Vector Name:: PPRE-H2B-eGFP

Antibiotic Resistance:: Kanamycin

Length:: 5664 bp

Type:: Protein expression

Replication origin:: ori

Host:: Mammalian cells

Selection Marker:: Neo/G418

Promoter:: CMV

5' Primer:: ForwardleftITR

Growth Strain(s):: DH5a

Growth Temperature:: 37℃

PPRE-H2B-eGFP vector Map

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PPRE-H2B-eGFP vector Sequence

LOCUS       .                       5664 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1610..2326
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     polyA_signal    2452..2573
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2580..3035)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3062..3166
                     /label="AmpR promoter"
     promoter        3168..3525
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3560..4351
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    4586..4633
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4962..5550
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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