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mScarlet-EEA1 vector (V017581)

Basic Vector Information

Vector Name:
mScarlet-EEA1
Antibiotic Resistance:
Kanamycin
Length:
8907 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Selection Marker:
Neo/G418
Promoter:
CMV
3' Primer:
Sv40-polyA-R
Growth Strain(s):
DH5a
Growth Temperature:
37℃

mScarlet-EEA1 vector Map

Copy Sequence View Map
mScarlet-EEA18907 bp40080012001600200024002800320036004000440048005200560060006400680072007600800084008800CMV enhancerCMV promotermScarletEEA1SV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRHSV TK poly(A) signalori

mScarlet-EEA1 vector Sequence

LOCUS       .                       8907 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        98..401
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        402..605
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             650..1345
                     /label="mScarlet"
                     /note="bright monomeric red fluorescent protein evolved
                     from a synthetic template (Bindels et al., 2016)"
     CDS             1367..5599
                     /label="EEA1"
                     /note="human early endosome antigen 1"
     polyA_signal    5732..5853
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5860..6315)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6342..6446
                     /label="AmpR promoter"
     promoter        6448..6805
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             6840..7631
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    7866..7913
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8242..8830
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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