Basic Vector Information
- Vector Name:
- pCMV-mEGFP-MCS-HA-Neo
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4751 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- CMV
- 5' Primer:
- EGFP-F3
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pCMV-mEGFP-MCS-HA-Neo vector Map
pCMV-mEGFP-MCS-HA-Neo vector Sequence
LOCUS . 4751 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
enhancer 61..364
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1329
/label="mEGFP"
/note="enhanced GFP with monomerizing A206K mutation
(Zacharias et al., 2002)"
CDS 1381..1407
/label="HA"
/note="HA (human influenza hemagglutinin) epitope tag"
polyA_signal 1539..1660
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1667..2122)
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2149..2253
/label="AmpR promoter"
promoter 2255..2612
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 2647..3438
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase from Tn5"
polyA_signal 3673..3720
/label="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4049..4637
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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