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pZS42-pLlacO1-MCS vector (V017527)

Basic Vector Information

Vector Name:
pZS42-pLlacO1-MCS
Antibiotic Resistance:
Spectinomycin
Length:
3805 bp
Type:
Protein expression
Replication origin:
pSC101 ori
Host:
E. coli
Copy Number:
Low
3' Primer:
pQE-R
Growth Strain(s):
DH5a
Growth Temperature:
30℃

pZS42-pLlacO1-MCS vector Map

Copy Sequence View Map
pZS42-pLlacO1-MCS3805 bp60012001800240030003600lac operatorrrnB T1 terminatorRep101pSC101 orilambda t0 terminatorSmR

pZS42-pLlacO1-MCS vector Sequence

LOCUS       .                       3805 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    9..25
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     terminator      123..209
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(747..1694)
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     rep_origin      complement(1742..1964)
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2456..2550)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2622..3410)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"

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