Basic Vector Information
- Vector Name:
- AatII-pRPS5A-PTP-3Xflag-rbc L 294 Right_G1397 C-ABE
- Antibiotic Resistance:
- Spectinomycin
- Length:
- 13148 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Plants
- Selection Marker:
- Basta
- 5' Primer:
- Forward(left ITR)
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
AatII-pRPS5A-PTP-3Xflag-rbc L 294 Right_G1397 C-ABE vector Map
AatII-pRPS5A-PTP-3Xflag-rbc L 294 Right_G1397 C-ABE vector Sequence
LOCUS . 13148 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
protein_bind complement(5..25)
/label="attB4"
/note="core recombination site for the Gateway® BP
reaction"
CDS 1857..1922
/label="3xFLAG"
/note="three tandem FLAG® epitope tags, followed by an
enterokinase cleavage site"
misc_feature 5335..5359
/label="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
CDS 6690..7316
/label="pVS1 StaA"
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 7748..8818
/label="pVS1 RepA"
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 8887..9081
/label="pVS1 oriV"
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 9425..9565
/label="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(9751..10339)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10588..11376)
/label="SmR"
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
misc_feature 11897..11921
/label="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
terminator complement(12014..12266)
/label="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(12289..12837)
/label="BlpR"
/note="phosphinothricin acetyltransferase"
promoter complement(12879..13058)
/label="NOS promoter"
/note="nopaline synthase promoter"
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