pYM35 vector (V017506)

LOCUS       Exported                4866 bp DNA     circular SYN 22-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   P30247
VERSION     .
KEYWORDS    pYM35
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Self
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished."
COMMENT     A versatile toolbox for PCR-based tagging of yeast genes: new 
            fluorescent proteins, more markers and promoter substitution 
            cassettes. Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, 
            Maekawa H, Moreno-Borchart A, Doenges G, Schwob E, Schiebel E,
            Knop M. Yeast (2004) 21, 947 - 962
FEATURES             Location/Qualifiers
     source          1..4866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             79..759
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     terminator      781..968
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            1043..2399
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1043..1386
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1387..2196
                     /gene="kanMX"
                     /label=kanMX
     terminator      2202..2399
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(2504..2522)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2780..3368)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3539..4399)
                     /gene="AmpR"
                     /label=AmpR
     promoter        complement(4400..4504)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(4850..4866,1..2)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"