Basic Vector Information
pegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installation
- Vector Name:
- AAVS1 pegRNA1 with trimmed attP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 2368 bp
- Type:
- CRISPR Plasmids
- Source/Author:
- David Liu
- Copy Number:
- High copy number
- Promoter:
- U6
- Growth Strain(s):
- DH5alpha
- Growth Temperature:
- 37℃
AAVS1 pegRNA1 with trimmed attP vector Map
AAVS1 pegRNA1 with trimmed attP vector Sequence
LOCUS Exported 2368 bp DNA circular SYN 06-SEP-2024 DEFINITION pegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installation. ACCESSION . VERSION . KEYWORDS AAVS1 pegRNA1 with trimmed attP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2368) AUTHORS Pandey S, Gao XD, Krasnow NA, McElroy A, Tao YA, Duby JE, Steinbeck BJ, McCreary J, Pierce SE, Tolar J, Meissner TB, Chaikof EL, Osborn MJ, Liu DR TITLE Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime editing. JOURNAL Nat Biomed Eng. 2024 Jun 10. doi: 10.1038/s41551-024-01227-1. PUBMED 38858586 REFERENCE 2 (bases 1 to 2368) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed Eng. 2024 Jun 10. doi: 10.1038/s41551-024-01227-1." FEATURES Location/Qualifiers source 1..2368 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 340..359 /label=pBR322ori-F /note="pBR322 origin, forward primer" misc_RNA complement(632..707) /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" promoter complement(728..976) /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)" primer_bind complement(786..805) /label=LKO.1 5' /note="Human U6 promoter, forward primer" primer_bind complement(956..976) /label=hU6-F /note="Human U6 promoter, forward primer" promoter 1083..1187 /gene="bla" /label=AmpR promoter CDS 1188..2048 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1406..1425) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin join(2219..2368,1..439) /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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