pFBDM vector (V017482)

Basic Vector Information

      • Vector Name:
      • pFBDM
      • Antibiotic Resistance:
      • Ampicillin, Gentamycin
      • Length:
      • 5279 bp
      • Type:
      • Protein expression
      • Replication origin:
      • ori
      • Host:
      • Insect cells
      • Selection Marker:
      • Gen
      • Promoter:
      • p10
      • Growth Strain(s):
      • DH5a
      • Growth Temperature:
      • 37℃

pFBDM vector Vector Map

pFBDM5279 bp6001200180024003000360042004800f1 oriAmpR promoterAmpRoriTn7RGmRPc promoterHSV TK poly(A) signalp10 promoterpolyhedrin promoterSV40 poly(A) signalTn7L

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pFBDM vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_10810        5279 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      106..560
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        586..690
                     /label=AmpR promoter
     CDS             691..1548
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1722..2310
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(2615..2839)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2909..3439)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(3628..3656)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    complement(4185..4233)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(4369..4478)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        4523..4614
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    4873..5007
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(5036..5201)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"

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