Basic Vector Information
- Vector Name:
- pEIGW Grx1-roGFP2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9865 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Promoter:
- SV40
- 5' Primer:
- pEGFP-N-3'
- Growth Temperature:
- 37℃
pEIGW Grx1-roGFP2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pEIGW Grx1-roGFP2 vector Sequence
LOCUS 62056_9125 9865 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9865)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..9865
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1914..1955
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
misc_feature 2032..2149
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2206..2417
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 2885..3598
/label=EGFP
/note="enhanced GFP"
primer_bind complement(3665..3681)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 3695..4283
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
protein_bind complement(4427..4460)
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
promoter complement(4685..4703)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
promoter 5491..5595
/label=AmpR promoter
CDS 5596..6453
/label=AmpR
/note="beta-lactamase"
rep_origin 6627..7215
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 7461..7790
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
intron 8924..8989
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 9119..9139
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 9564..9698
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
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