Basic Vector Information
- Vector Name:
- CMV-enAsCas12f
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4776 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- CMV
- 5' Primer:
- CMV-F
- Growth Temperature:
- 37℃
CMV-enAsCas12f vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
CMV-enAsCas12f vector Sequence
LOCUS 62056_551 4776 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4776)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..4776
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(62..919)
/label=AmpR
/note="beta-lactamase"
promoter complement(920..1024)
/label=AmpR promoter
enhancer 1294..1673
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1674..1877
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 1919..1937
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1987..2007
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 2008..3273
/gene="cas12f1"
/label=cas12f1
/note="CRISPR-associated endodeoxyribonuclease Cas12f1 from
Sulfoacidibacillus thermotolerans. Accession#: A0A2U3D0N8"
CDS 3304..3324
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 3333..3350
/label=6xHis
/note="6xHis affinity tag"
polyA_signal 3379..3603
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(3674..3690)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(3698..3714)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3722..3752)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(3767..3788)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4076..4664)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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