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pEGFP Tensin3 vector (V017369)

Basic Vector Information

Vector Name:
pEGFP Tensin3
Antibiotic Resistance:
Kanamycin
Length:
9055 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Selection Marker:
Neo/G418
Promoter:
CMV
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pEGFP Tensin3 vector Vector Map

pEGFP Tensin39055 bp40080012001600200024002800320036004000440048005200560060006400680072007600800084008800oriCMV enhancerCMV promoterEGFPTensin-3SV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRHSV TK poly(A) signal

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pEGFP Tensin3 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V017369                 9055 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017369
VERSION     V017369
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..594
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     enhancer        758..1061
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1062..1265
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1310..2026
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             2063..6397
                     /gene="TNS3"
                     /label="Tensin-3"
                     /note="Tensin-3 from Homo sapiens. Accession#: Q68CZ2"
     polyA_signal    6551..6672
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(6679..7134)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7161..7265
                     /label="AmpR promoter"
     promoter        7267..7624
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             7659..8450
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    8685..8732
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"

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