Basic Vector Information
- Vector Name:
- CaV2.1 (pcDNA6)
- Antibiotic Resistance:
- Ampicillin
- Length:
- 12495 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Blast
- Promoter:
- SV40
- 5' Primer:
- CMV-F
- Growth Temperature:
- 37℃
CaV2.1 (pcDNA6) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
CaV2.1 (pcDNA6) vector Sequence
LOCUS 62056_486 12495 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12495) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..12495 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 629..647 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 755..7273 /gene="Cacna1a" /label=Cacna1a /note="Voltage-dependent P/Q-type calcium channel subunit alpha-1A from Rattus norvegicus. Accession#: P54282" CDS 8126..8167 /label=V5 tag /note="epitope tag from simian virus 5" CDS 8177..8194 /label=6xHis /note="6xHis affinity tag" polyA_signal 8223..8447 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 8493..8921 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 8935..9264 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 9312..9359 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 9378..9773 /label=BSD /note="blasticidin S deaminase" promoter complement(10152..10182) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(10197..10218) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(10506..11094) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(11268..12125) /label=AmpR /note="beta-lactamase" promoter complement(12126..12230) /label=AmpR promoter
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