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pSIN REP5-GFP-GluR2 (Q) vector (V017323)

Price Information

Cat No. Plasmid Name Availability Add to cart
V017323 pSIN REP5-GFP-GluR2 (Q) In stock, 1 week for quality controls

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Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.

Basic Vector Information

Vector Name:
pSIN REP5-GFP-GluR2 (Q)
Antibiotic Resistance:
Ampicillin
Length:
13753 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Growth Temperature:
37℃

pSIN REP5-GFP-GluR2 (Q) vector Map

Copy Sequence View Map
pSIN REP5-GFP-GluR2 (Q)13753 bp600120018002400300036004200480054006000660072007800840090009600102001080011400120001260013200EGFPGlutamate receptor 2AmpR promoterAmpRori

Plasmid Protocol

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5. Store the plasmid at -20 ℃.

6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it

General Plasmid Transform Protocol

1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.

2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.

3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.

4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.

5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.

pSIN REP5-GFP-GluR2 (Q) vector Sequence

LOCUS       V017323                13753 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017323
VERSION     V017323
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13753)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             8069..8782
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             8783..11365
                     /gene="Gria2"
                     /label="Glutamate receptor 2"
                     /note="Glutamate receptor 2 from Rattus norvegicus.
                     Accession#: P19491"
     promoter        11924..12028
                     /label="AmpR promoter"
     CDS             12029..12886
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      13060..13648
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"