Basic Vector Information
- Vector Name:
- pENO1-iRFP-NATr
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6064 bp
- Type:
- Gene expression
- Replication origin:
- ori
- Host:
- Candida albicans
- Selection Marker:
- NAT
- Promoter:
- TEF
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pENO1-iRFP-NATr vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pENO1-iRFP-NATr vector Sequence
LOCUS 62056_9450 6064 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6064)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6064
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 450..1382
/codon_start=1
/label=iRFP670
/note="near-infrared fluorescent protein with an emission
peak at 670 nm, engineered from a bacterial phytochrome
(Shcherbakova and Verkhusha, 2013)"
/translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
terminator 1405..1592
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
promoter 1664..2007
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
CDS 2014..2577
/codon_start=1
/label=NrsR
/note="nourseothricin acetyltransferase"
/translation="TTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFTL
REVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRRL
TVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTLC
GLDTALYDGTASDGEQALYMSMPCP"
terminator 2586..2783
/label=TEF terminator
/note="Ashbya gossypii TEF terminator"
primer_bind complement(2873..2889)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(2897..2913)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2921..2951)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(2966..2987)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3275..3863)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4037..4894)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4895..4999)
/label=AmpR promoter
primer_bind 5473..5489
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
This page is informational only.