Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V017256 | pBEN276 | In stock, 1 week for quality controls |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pBEN276
- Antibiotic Resistance:
- Ampicillin
- Length:
- 19671 bp
- Type:
- Signal transduction
- Replication origin:
- pSC101 ori
- Host:
- Gram-negative bacteria
- Copy Number:
- Low
- Promoter:
- araBAD
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 30℃
pBEN276 vector Vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pBEN276 vector Sequence
LOCUS V017256 19671 bp DNA circular SYN 01-JAN-1980
DEFINITION Exported.
ACCESSION V017256
VERSION V017256
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 19671)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..19671
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 379..1818
/label="LuxC"
/note="LuxC fatty acid reductase"
CDS 2934..4010
/label="LuxA"
/note="LuxA luciferase subunit"
primer_bind complement(6265..6281)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(6289..6305)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6313..6343)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(6358..6379)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
mobile_element complement(7230..7395)
/label="Tn7L"
/note="mini-Tn7 element (left end of the Tn7 transposon)"
CDS complement(7831..8778)
/label="Rep101(Ts)"
/note="temperature-sensitive version of the RepA protein
needed for replication with the pSC101 origin (Armstrong et
al., 1984)"
rep_origin complement(8826..9048)
/direction=LEFT
/label="pSC101 ori"
/note="low-copy replication origin that requires the Rep101
protein"
oriT 9788..9897
/label="oriT"
/note="incP origin of transfer"
CDS complement(10071..10928)
/label="AmpR"
/note="beta-lactamase"
promoter complement(10929..11020)
/label="AmpR promoter"
terminator complement(11039..11066)
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator complement(11158..11244)
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(13410..15074)
/gene="tnsC"
/label="Transposon Tn7 transposition protein TnsC"
/note="Transposon Tn7 transposition protein TnsC from
Escherichia coli. Accession#: P05846"
CDS complement(15074..17179)
/gene="tnsB"
/label="Transposon Tn7 transposition protein TnsB"
/note="Transposon Tn7 transposition protein TnsB from
Escherichia coli. Accession#: P13989"
CDS complement(17169..17987)
/gene="tnsA"
/label="Transposon Tn7 transposition protein TnsA"
/note="Transposon Tn7 transposition protein TnsA from
Escherichia coli. Accession#: P13988"
promoter complement(18105..18389)
/label="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
CDS 18416..19291
/label="araC"
/note="L-arabinose regulatory protein"