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pEGFP-VSP4-E228Q vector (V017238)

Basic Vector Information

Vector Name:
pEGFP-VSP4-E228Q
Antibiotic Resistance:
Kanamycin
Length:
6024 bp
Type:
Gene expression
Replication origin:
ori
Host:
Mammalian cells
Selection Marker:
Neo/G418
Promoter:
CMV
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pEGFP-VSP4-E228Q vector Vector Map

pEGFP-VSP4-E228Q6024 bp30060090012001500180021002400270030003300360039004200450048005100540057006000CMV enhancerCMV promoterEGFPVacuolar protein sorting-associated protein 4ASV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRHSV TK poly(A) signalori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pEGFP-VSP4-E228Q vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V017238                 6024 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017238
VERSION     V017238
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             1369..2679
                     /gene="VPS4A"
                     /label="Vacuolar protein sorting-associated protein 4A"
                     /note="Vacuolar protein sorting-associated protein 4A from
                     Homo sapiens. Accession#: Q9UN37"
     polyA_signal    2812..2933
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2940..3395)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3422..3526
                     /label="AmpR promoter"
     promoter        3528..3885
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3920..4711
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4946..4993
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5322..5910
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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