Basic Vector Information
- Vector Name:
- pET-21a(+)-IPP1-His
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6228 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- E. coli
- Promoter:
- T7
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pET-21a(+)-IPP1-His vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pET-21a(+)-IPP1-His vector Sequence
LOCUS V017199 6228 bp DNA circular SYN 01-JAN-1980 DEFINITION Exported. ACCESSION V017199 VERSION V017199 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 6228) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..6228 /mol_type="other DNA" /organism="synthetic DNA construct" promoter complement(1009..1086) /label="lacI promoter" promoter 1399..1417 /label="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 1418..1442 /label="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 1457..1479 /label="RBS" /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 1487..2347 /gene="IPP1" /label="Inorganic pyrophosphatase" /note="Inorganic pyrophosphatase from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). Accession#: P00817" CDS 2354..2371 /label="6xHis" /note="6xHis affinity tag" terminator 2438..2485 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 2522..2977 /label="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3003..3107 /label="AmpR promoter" CDS 3108..3965 /label="AmpR" /note="beta-lactamase" rep_origin 4139..4727 /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(4913..5052) /label="bom" /note="basis of mobility region from pBR322" CDS complement(5157..5345) /label="rop" /note="Rop protein, which maintains plasmids at low copy number" protein_bind complement(6120..6141) /label="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." CDS complement(join(6157..6228,1..1008)) /label="lacI" /note="lac repressor"
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