Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V017022 | pX037 | In stock, 1 week for quality controls |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pX037
- Antibiotic Resistance:
- Kanamycin
- Length:
- 19895 bp
- Type:
- Protein expression
- Replication origin:
- oriV
- Host:
- Plants
- Selection Marker:
- Neo/G418
- Promoter:
- CaMV 35S
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pX037 vector Vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pX037 vector Sequence
LOCUS V017022 19895 bp DNA circular SYN 01-JAN-1980
DEFINITION Exported.
ACCESSION V017022
VERSION V017022
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 19895)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..19895
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 749..1894
/label="trfA"
/note="trans-acting replication protein that binds to and
activates oriV"
misc_feature 2043..2067
/label="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
promoter 2230..2409
/label="NOS promoter"
/note="nopaline synthase promoter"
misc_feature 2440..2494
/label="TMV Omega"
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
CDS 2496..3287
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
terminator 3317..4022
/label="OCS terminator"
/note="octopine synthase terminator"
promoter 4124..4469
/label="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
promoter 10772..10818
/label="minimal CaMV 35S promoter"
/note="minimal 35S promoter from cauliflower mosaic virus"
misc_feature 10849..10902
/label="TMV Omega"
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
CDS 10911..12176
/gene="h3h"
/label="Hispidin-3-hydroxylase"
/note="Hispidin-3-hydroxylase from Neonothopanus nambi.
Accession#: A0A3G9K5C8"
CDS 13434..14234
/gene="luz"
/label="Luciferase"
/note="Luciferase from Neonothopanus nambi. Accession#:
A0A3G9JYH7"
CDS 14241..14258
/label="6xHis"
/note="6xHis affinity tag"
terminator 14286..14993
/label="OCS terminator"
/note="octopine synthase terminator"
promoter 15098..15443
/label="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
protein_bind 17242..17263
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 17278..17308
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 17316..17332
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 17340..17356
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
misc_feature complement(17369..17425)
/label="MCS"
/note="pUC18/19 multiple cloning site"
misc_feature 17807..17831
/label="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
rep_origin 17918..18629
/label="oriV"
/note="incP origin of replication"
promoter 18650..18754
/label="AmpR promoter"
CDS 18755..19546
/label="KanR"
/note="aminoglycoside phosphotransferase"