Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V016803 | pZSm45 ndhII | In stock, 1 week for quality controls |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pZSm45 ndhII
- Antibiotic Resistance:
- Streptomycin
- Length:
- 5793 bp
- Type:
- Protein expression
- Replication origin:
- pSC101 ori
- Host:
- E. coli
- Promoter:
- pLux
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 30℃
pZSm45 ndhII vector Vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pZSm45 ndhII vector Sequence
LOCUS V016803 5793 bp DNA circular SYN 01-JAN-1980
DEFINITION Exported.
ACCESSION V016803
VERSION V016803
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 5793)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..5793
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(4..753)
/gene="luxR"
/label="Transcriptional activator protein LuxR"
/note="Transcriptional activator protein LuxR from
Aliivibrio fischeri. Accession#: P12746"
CDS 980..2281
/gene="ndh"
/label="Type II NADH:quinone oxidoreductase"
/note="Type II NADH:quinone oxidoreductase from Escherichia
coli (strain K12). Accession#: P00393"
CDS 2282..2314
/label="ssrA tag"
/note="C-terminal peptide that mediates degradation in
bacteria through the ClpXP and ClpAP proteases (McGinness
et al., 2006)"
terminator 2335..2421
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin 2920..3142
/label="pSC101 ori"
/note="low-copy replication origin that requires the Rep101
protein"
CDS 3190..4137
/label="Rep101"
/note="RepA protein needed for replication with the pSC101
origin"
terminator complement(4671..4765)
/label="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS complement(4780..5568)
/label="SmR"
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"