Basic Vector Information
- Vector Name:
- pEGFP-AQP9(human)
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5610 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- CMV
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pEGFP-AQP9(human) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pEGFP-AQP9(human) vector Sequence
LOCUS V016798 5610 bp DNA circular SYN 01-JAN-1980 DEFINITION Exported. ACCESSION V016798 VERSION V016798 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 5610) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..5610 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 61..364 /label="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" CDS 613..1329 /label="EGFP" /note="enhanced GFP" CDS 1381..2265 /gene="AQP9" /label="Aquaporin-9" /note="Aquaporin-9 from Homo sapiens. Accession#: O43315" polyA_signal 2398..2519 /label="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(2526..2981) /direction=LEFT /label="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3008..3112 /label="AmpR promoter" promoter 3114..3471 /label="SV40 promoter" /note="SV40 enhancer and early promoter" CDS 3506..4297 /label="NeoR/KanR" /note="aminoglycoside phosphotransferase" polyA_signal 4532..4579 /label="HSV TK poly(A) signal" /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4908..5496 /direction=RIGHT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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