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pGTMV vector (V016602)

Basic Vector Information

This vector seems to be unstable. The Sanger sequencing was correct, but the restriction digestion was not. There is no good signal from the third-generation sequencing.

Vector Name:
pGTMV
Antibiotic Resistance:
Kanamycin
Length:
9669 bp
Type:
Protein expression
Replication origin:
pSa ori
Host:
Plants
Copy Number:
Low
Promoter:
35s
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pGTMV vector Map

Copy Sequence View Map
pGTMV9669 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400880092009600ReplicaseReplicaseCapsid proteinRB T-DNA repeatoriKanRpSa oriLB T-DNA repeat

pGTMV vector Sequence

LOCUS       Exported                9669 bp DNA     circular SYN 05-SEP-2024
DEFINITION  Exported.
ACCESSION   V016602
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9669)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             20..3508
                     /codon_start=1
                     /product="Replicase [Tobacco mosaic virus]"
                     /label=Replicase
                     /translation="MTHNPTILRKTLPLYKEVHFIWRGIFTTITNNNKQQTTLQLLFTI
                     TMAYTQTATTSALLDTVRGNNSLVNDLAKRRLYDTAVEEFNARDRRPKVNFSKVISEEQ
                     TLIATRAYPEFQITFYNTQNAVHSLAGGLRSLELEYLMMQIPYGSLTYDIGGNFASHLF
                     KGRAYVHCCMPNLDVRDIMRHEGQKDSIELYLSRLERGGKTVPNFQKEAFDRYAEIPED
                     AVCHNTFQTCEHQPMQQSGRVYAIALHSIYDIPADEFGAALLRKNVHTCYAAFHFSENL
                     LLEDSCVNLDEINACFSRDGDKLTFSFASESTLNYCHSYSNILKYVCKTYFPASNREVY
                     MKEFLVTRVNTWFCKFSRIDTFLLYKGVAHKSVDSEQFYTAMEDAWHYKKTLAMCNSER
                     ILLEDSSSVNYWFPKMRDMVIVPLFDISLETSKRTRKEVLVSKDFVFTVLNHIRTYQAK
                     ALTYANVLSFVESIRSRVIINGVTARSEWDVDKSLLQSLSMTFYLHTKLAVLKDDLLIS
                     KFSLGSKTVCQHVWDEISLAFGNAFPSVKERLLNRKLIRVAGDALEIRVPDLYVTFHDR
                     LVTEYKASVDMPALDIRKKMEETEVMYNALSELSVLRESDKFDVDVFSQMCQSLEVDPM
                     TAAKVIVAVMSNESGLTLTFERPTEANVALALQDQEKASEGALVVTSREVEEPSMKGSM
                     ARGELQLAGLAGDHPESSYSKNEEIESLEQFHMATADSLIRKQMSSIVYTGPIKVQQMK
                     NFIDSLVASLSAAVSNLVKILKDTAAIDLETRQKFGVLDVASRKWLIKPTAKSHAWGVV
                     ETHARKYHVALLEYDEQGVVTCDDWRRVAVSSESVVYSDMAKLRTLRRLLRNGEPHVSS
                     AKVVLVDGVPGCGKTKEILSRVNFDEDLILVPGKQAAEMIRRRANSSGIIVATKDNVKT
                     VDSFMMNFGKSTRCQFKRLFIDEGLMLHTGCVNFLVAMSLCEIAYVYGDTQQIPYINRV
                     SGFPYPAHFAKLEVDEVETRRTTLRCPADVTHYLNRRYEGFVMSTSSVKKSVSQEMVGG
                     AAVINPISKPLHGKILTFTQSDKEALLSRGYSDVHTVHEVQGETYSDVSLVRLTPTPVS
                     IIAGDSPHVLVALSRHTCSLKYYTVVMDPLVSIIRDLEKLSSYLLDMYKVDAGTQ"
     misc_feature    101..156
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             3584..5008
                     /codon_start=1
                     /product="Replicase [Tobacco mosaic virus]"
                     /label=Replicase
                     /translation="MQFYYDKCLPGNSTMMNNFDAVTMRLTDISLNVKDCILDMSKSVA
                     APKDQIKPLIPMVRTAAEMPRQTGLLENLVAMIKRNFNAPELSGIIDIENTASLVVDKF
                     FDSYLLKEKRKPNKNVSLFSRESLNRWLEKQEQVTIGQLADFDFVDLPAVDQYRHMIKA
                     QPKQKLDTSIQTEYPALQTIVYHSKKINAIFGPLFSELTRQLLDSVDSSRFLFFTRKTP
                     AQIEDFFGDLDSHVPMDVLELDISKYDKSQNEFHCAVEYEIWRRLGFEDFLGEVWKQGH
                     RKTTLKDYTAGIKTCIWYQRKSGDVTTFIGNTVIIAACLASMLPMEKIIKGAFCGDDSL
                     LYFPKGCEFPDVQHSANLMWNFEAKLFKKQYGYFCGRYVIHHDRGCIVYYDPLKLISKL
                     GAKHIKDWEHLEEFRRSLCDVAVSLNNCAYYTQLDDAVWEVHKTAPPGSFVYKSLVKYL
                     SDKVLFRSLFIDGSSC"
     CDS             4992..5795
                     /gene="MP"
                     /label=Movement protein
                     /note="Movement protein from Tobacco mosaic virus (strain 
                     vulgare). Accession#: P03583"
     CDS             5801..6277
                     /gene="CP"
                     /label=Capsid protein
                     /note="Capsid protein from Tobacco mosaic virus (strain 
                     vulgare). Accession#: P69687"
     misc_feature    6774..6798
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6917..7505)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7679..8491)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      8782..9217
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    9368..9392
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"

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