pPM65-lacZ vector (V016566)

LOCUS       V016566                 5443 bp    DNA     circular SYN 25-MAY-2021
DEFINITION  Exported.
ACCESSION   V016566
VERSION     V016566
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Mordaka PM, Heap JT.
  TITLE     Combinatorial assembly of integration-activated metabolic pathway
            libraries in Clostridium acetobutlicym
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Mordaka PM, Heap JT.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2020) Life Sciences, Imperial College London,
            Imperial College Road, London SW7 2AY, United Kingdom
REFERENCE   3  (bases 1 to 5443)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-APR-2020) Life Sciences, Imperial College London, Imperial
            College Road, London SW7 2AY, United Kingdom"
FEATURES             Location/Qualifiers
     source          1..5443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      9..62
                     /label="derived from CD0164 gene of Clostridium difficile"
                     /note="derived from CD0164 gene of Clostridium difficile"
                     /regulatory_class="terminator"
     primer_bind     63..79
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    90..389
                     /note="mediates homologous recombination with the
                     chromosome of Clostridium acetobutylicum ATCC824"
     CDS             413..1147
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     protein_bind    1192..1213
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1228..1258
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1266..1282
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    complement(1319..1375)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1376..1392)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    1643..2842
                     /note="mediates homologous recombination with the
                     chromosome of Clostridium acetobutylicum ATCC824"
     CDS             2078..2443
                     /codon_start=1
                     /transl_table=11
                     /product="CAC2872"
                     /label="CAC2872"
                     /note="putative FoF1-type ATP synthase operon membrane
                     protein"
                     /protein_id="QVN25285.1"
                     /translation="MNLNIKRMLKVVTLYDAIIAAIVSVILLFAANYKISLIVIIGIFS
                     AIFNFYLSNLTADFVFVKKMGNTSLIFLSSIFRVILVFFIGIILYKIYKYYLIAYLGGY
                     SAHFIALIIYGSLVNKR"
     regulatory      2843..2884
                     /label="TT2"
                     /note="TT2"
                     /regulatory_class="terminator"
     CDS             3263..3703
                     /codon_start=1
                     /transl_table=11
                     /product="RepL"
                     /label="RepL"
                     /note="plasmid replication protein"
                     /protein_id="QVN25286.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEID"
     CDS             3853..4473
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     rep_origin      4653..5241
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"