pTet-(33sug)TEV-(33teg)TVMV-(33tvg)SuMMV vector (V016557)

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LOCUS       62056_21140        4903 bp DNA     circular SYN 25-MAR-2019
DEFINITION  Cloning vector pTet-(33sug)TEV-(33teg)TVMV-(33tvg)SuMMV, complete 
            sequence.
ACCESSION   MK238520
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Gao C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2018) State Key Laboratory of Food Science and 
            Technology, Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 
            214122, China
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-NOV-2018) State Key Laboratory of Food Science and Technology, 
            Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 214122, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..633)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     promoter        649..704
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     regulatory      720..730
                     /label=B0033
                     /note="B0033"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    746..763
                     /label=summv protease site
                     /note="summv protease site"
     misc_feature    764..799
                     /label=beta gal degradation signal
                     /note="beta gal degradation signal"
     CDS             800..1501
                     /label=TEV protease
                     /note="tobacco etch virus protease (Kapust et al., 2001)"
     regulatory      1511..1521
                     /label=B0033
                     /note="B0033"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    1531..1548
                     /label=tev protease site
                     /note="tev protease site"
     misc_feature    1549..1584
                     /label=beta gal degradation signal
                     /note="beta gal degradation signal"
     misc_feature    1585..2238
                     /label=TVMVp
                     /note="TVMVp"
     regulatory      2245..2255
                     /label=B0033
                     /note="B0033"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    2265..2282
                     /label=TVMV protease site
                     /note="TVMV protease site"
     misc_feature    2283..2318
                     /label=beta gal degradation signal
                     /note="beta gal degradation signal"
     misc_feature    2319..3044
                     /label=SuMMVp
                     /note="SuMMVp"
     terminator      3068..3139
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3155..3182
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(3344..3889)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(4003..4097)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4121..4777)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(4778..4880)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"