MCPyV vector (V016552)

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LOCUS       V016552                 8704 bp    DNA     circular SYN 06-SEP-2021
DEFINITION  Exported.
ACCESSION   V016552
VERSION     V016552
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Kervarrec T, Cheret J, Paus R, Houben R, Schrama D.
  TITLE     Transduction-induced overexpression of Merkel cell T antigens in
            human hair follicles induces formation of pathological cell clusters
            with Merkel cell carcinoma-like phenotype
  JOURNAL   Exp Dermatol (2021) In press
   PUBMED   34386998
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   Schrama D, Houben R, Kervarrec T.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2021) Dermatology, University Hospital Wuerzburg,
            Josef-Schneider-Str. 2, Wuerzburg, Bavaria 97080, Deutschland
REFERENCE   3  (bases 1 to 8704)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Exp
            Dermatol (2021) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JUL-2021) Dermatology, University Hospital Wuerzburg,
            Josef-Schneider-Str. 2, Wuerzburg, Bavaria 97080, Deutschland"
FEATURES             Location/Qualifiers
     source          1..8704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             234..414
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1804..1920
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2010..2213
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             join(2299..2532,2964..3632)
                     /codon_start=1
                     /transl_table=11
                     /product="truncated MCPyV LT"
                     /label="truncated MCPyV LT"
                     /note="MCPyV LT 300"
                     /protein_id="QZR95626.1"
                     /translation="MDLVLNRKEREALCKLLEIAPNCYGNIPLMKAAFKRSCLKHHPDK
                     GGNPVIMMELNTLWSKFQQNIHKLRSDFSMFDEVDEAPIYGTTKFKEWWRSGGFSFGKA
                     YEYGPNPHGTNSRSRKPSSNASRGAPSGSSPPHSQSSSSGYGSFSASQASDSQSRGPDI
                     PPEHHEEPTSSSGSSSREETTNSGRESSTPNGTSVPRNSSRTDGTWEDLFCDESLSSPE
                     PPSSSEEPEEPPSSRSSPRQPPSSSAEEASSSQFTDEEYRSSSFTTPKTPPPFSRKRKF
                     GGSRSSASSASSASSTSTP"
     CDS             2299..2859
                     /codon_start=1
                     /transl_table=11
                     /product="small T antigen"
                     /label="small T antigen"
                     /note="MCPyV small T antigen; sT"
                     /protein_id="QZR95627.1"
                     /translation="MDLVLNRKEREALCKLLEIAPNCYGNIPLMKAAFKRSCLKHHPDK
                     GGNPVIMMELNTLWSKFQQNIHKLRSDFSMFDEVSTKFPWEEYGTLKDYMQSGYNARFC
                     RGPGCMLKQLRDSKCACISCKLSRQHCSLKTLKQKNCLTWGECFCYQCFILWFGFPPTW
                     ESFDWWQKTLEETDYCLLHLHLF"
     promoter        3674..3885
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     LTR             3898..4166
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4193..4789
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    4799..5387
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5461..5694
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5766..5900
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5906..6041
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(6079..6095)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6103..6119)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6127..6157)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6172..6193)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6481..7069)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7243..8100)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8101..8205)
                     /label="AmpR promoter"
     primer_bind     8679..8695
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"