pSaBIN2 vector (V016551)

Basic Vector Information

Vector Name:
pSaBIN2
Antibiotic Resistance:
Chloramphenicol
Length:
3160 bp
Type:
Binary vector
Replication origin:
pSa ori
Source/Author:
Banno H.

pSaBIN2 vector Map

pSaBIN23160 bp6001200180024003000CmRoriRB T-DNA repeatmultiple cloning sitesLB T-DNA repeatpSa ori

pSaBIN2 vector Sequence

LOCUS       62056_19175        3160 bp DNA     circular SYN 14-OCT-2021
DEFINITION  Binary vector pSaBIN2 DNA, complete sequence.
ACCESSION   LC652997
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Banno H.
  TITLE     Plant transient expression vector pSaBIN
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Banno H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2021) Contact:Hiroharu Banno Chubu University, 
            College of Bioscience & Biotechnology; 1200 Matsumoto-cho, Kasugai, 
            Aichi 4878501, Japan
REFERENCE   3  (bases 1 to 3160)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-OCT-2021) Contact:Hiroharu Banno Chubu University, College of 
            Bioscience & Biotechnology; 1200 Matsumoto-cho, Kasugai, Aichi 
            4878501, Japan"
FEATURES             Location/Qualifiers
     source          1..3160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             250..906
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     rep_origin      1072..1660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2161..2185)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2196..2246
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     misc_feature    complement(2298..2322)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2681..3116)
                     /direction=LEFT
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"

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