pCW619-LI-dat vector (V016545)

Basic Vector Information

Vector Name:
pCW619-LI-dat
Antibiotic Resistance:
Ampicillin
Length:
8118 bp
Type:
UNVERIFIED: Cloning vector
Replication origin:
ori
Source/Author:
Yuzhen Z.

pCW619-LI-dat vector Map

pCW619-LI-dat8118 bp400800120016002000240028003200360040004400480052005600600064006800720076008000ropbomoriAmpRAmpR promoterrRNA adenine N-6-methyltransferase

pCW619-LI-dat vector Sequence

LOCUS       V016545                 8118 bp    DNA     circular SYN 20-JUN-2020
DEFINITION  Exported.
ACCESSION   V016545
VERSION     V016545
KEYWORDS    UNVERIFIED
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Yuzhen Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2019) Department of Public Health Laboratory
            Sciences, West China School of Public HealthSichuan University, No.
            17, Section 3, Renmin South Road, Wuhou District, Chengdu, Sichuan
            610041, China
REFERENCE   2  (bases 1 to 8118)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (02-OCT-2019) Department of Public Health Laboratory Sciences, West
            China School of Public HealthSichuan University, No. 17, Section 3,
            Renmin South Road, Wuhou District, Chengdu, Sichuan 610041, China"
            GenBank staff is unable to verify sequence and/or annotation
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..8118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             515..703
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    808..948
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1134..1722)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1896..2753)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2754..2858)
                     /label="AmpR promoter"
     CDS             5798..6529
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"

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