pCI-650NS1-Gluc1 vector (V016524)

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LOCUS       V016524                 5019 bp    DNA     circular SYN 28-OCT-2020
DEFINITION  Exported.
ACCESSION   V016524
VERSION     V016524
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Declercq M, Biquand E, Karim M, Pietrosemoli N, Jacob Y, Demeret C,
            Barbezange C, van der Werf S.
  TITLE     Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to
            promote viral transcription
  JOURNAL   Nucleic Acids Res 48 (18), 10428-10440 (2020)
   PUBMED   32960265
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Declercq M, Biquand E, Demeret C, Karim M, Pietrosemoli N, Jacob Y,
            Demeret C, Van der Werf S, Barbezange C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2020) National Influenza Centre, Sciensano, Rue
            Juliette Wytsman 14, Brussels 1180, Belgium
REFERENCE   3  (bases 1 to 5019)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res"; date: "2020"; volume: "48"; issue: "18"; pages:
            "10428-10440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-SEP-2020) National Influenza Centre, Sciensano, Rue Juliette
            Wytsman 14, Brussels 1180, Belgium"
FEATURES             Location/Qualifiers
     source          1..5019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     misc_feature    1012..1027
                     /label="sequencing primer"
                     /note="sequencing primer"
     promoter        1034..1052
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1067..1756
                     /gene="NS"
                     /label="Non-structural protein 1"
                     /note="Non-structural protein 1 from Influenza A virus
                     (strain A/USA:Texas/UR06-0195/2007 H1N1). Accession#:
                     A8C8K0"
     misc_feature    1757..1795
                     /label="linker"
                     /note="linker"
     misc_feature    1796..2077
                     /label="Gaussia luciferase domain 1"
                     /note="Gaussia luciferase domain 1"
     polyA_signal    complement(2133..2254)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      2435..2890
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3222..3326
                     /label="AmpR promoter"
     CDS             3327..4184
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4358..4946
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"