pGHKW4 vector (V016518)

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LOCUS       62056_11565        5494 bp DNA     circular SYN 03-JUL-2019
DEFINITION  Cloning vector pGHKW4, complete sequence.
ACCESSION   MK562404
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Chenyan H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2019) Fujian Agriculture and Forestry University, 
            College of Forestry, No. 15 Shangdian Road, Cangshan District, 
            Fuzhou, Fujian 350002, China
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (21-FEB-2019) Fujian Agriculture and Forestry University, College of
            Forestry, No. 15 Shangdian Road, Cangshan District, Fuzhou, Fujian 
            350002, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      414..778
                     /note="nptII; promoter of transference"
                     /regulatory_class="promoter"
     CDS             824..1537
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     CDS             2469..3248
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(3273..3289)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3297..3313)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3321..3351)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3366..3387)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3675..4263)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4437..5294)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5295..5399)
                     /label=AmpR promoter