pBlu10-Htk vector (V016514)

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LOCUS       62056_4115        5756 bp DNA     circular SYN 30-SEP-2020
DEFINITION  Shuttle expression vector pBlu10-Htk, complete sequence.
ACCESSION   MN843970
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Le Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2019) School of Environment and Safety 
            Engineering, Jiangsu University, Xuefu Road 301, Zhen Jiang, Jiangsu
            212013, China
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2019) School of Environment and Safety Engineering, Jiangsu 
            University, Xuefu Road 301, Zhen Jiang, Jiangsu 212013, China"
FEATURES             Location/Qualifiers
     source          1..5756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      130..2505
                     /label=Gram-positive origin of replication
                     /note="Gram-positive origin of replication"
     regulatory      2506..3047
                     /regulatory_class="promoter"
     gene            3048..3809
                     /gene="Htk"
                     /label=Htk
     CDS             3048..3809
                     /codon_start=1
                     /transl_table=11
                     /gene="Htk"
                     /product="kanamycin resistance protein"
                     /label=Htk
                     /protein_id="QOC69212.1"
                     /translation="MKGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGRQTD
                     GPYSDIEMMCVLSTEGVEFSYEWTTGEWKAEVNFYSEEILLDYASRVEPDWPLTHGRFF
                     SILPIYDPGGYFEKVYQTAKSVEAQKFHDAICALIVEELFEYAGKWRNIRVQGPTTFLP
                     SLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQPDLPPGYVQLCQLVMSGQLSDPEK
                     LLESLENFWNGVQEWAERHGYIVDVSKRIPF"
     regulatory      3810..3832
                     /label=RBS
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    3836..3865
                     /label=MCS
                     /note="MCS"
     regulatory      3866..3923
                     /regulatory_class="terminator"
     rep_origin      complement(4009..4597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4771..5628)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5629..5733)
                     /label=AmpR promoter