pDEST17-AceKMs vector (V016507)

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LOCUS       V016507                 5842 bp    DNA     circular SYN 12-DEC-2019
DEFINITION  Exported.
ACCESSION   V016507
VERSION     V016507
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (10-MAY-2019) Biocatalysis "
FEATURES             Location/Qualifiers
     source          1..5842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..39
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             71..93
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             113..130
                     /label="6xHis"
                     /note="6xHis affinity tag"
     protein_bind    140..164
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             170..1297
                     /gene="ackA"
                     /label="Acetate kinase"
                     /note="Acetate kinase from Mycolicibacterium smegmatis
                     (strain ATCC 700084 / mc(2)155). Accession#: A0QQK1"
     protein_bind    complement(1308..1332)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      1399..1446
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(1812..1840)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        1953..2057
                     /label="AmpR promoter"
     CDS             2058..2915
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3089..3677
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(3863..4005)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(4110..4298)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"