yy630 vector (V016503)

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LOCUS       62056_23520        9323 bp DNA     circular SYN 08-SEP-2020
DEFINITION  LUC binary vector yy630 DNA, complete sequence.
ACCESSION   LC578834
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Yamamoto YY, Zhao CR.
  TITLE     The yy630 vector was constructed by replacing the LUC+ gene in the 
            yy621 vector with the Nluc' gene of Promega pNL1.1[Nluc] vector. The
            Nluc' gene is formed by replacing the initiation codon ATG of Nluc 
            gene with the ATGGCTATGGCT sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   Yamamoto YY, Zhao CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2020) Contact:Cheng-Ri Zhao Agricultural college 
            of Yanbian University, Department of horticulture & landscape 
            architecture; No.977, Gongyuan Road, Yanji, Jilin Province 133002, 
            China
REFERENCE   3  (bases 1 to 9323)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2020) Contact:Cheng-Ri Zhao Agricultural college of Yanbian 
            University, Department of horticulture & landscape architecture; 
            No.977, Gongyuan Road, Yanji, Jilin Province 133002, China"
FEATURES             Location/Qualifiers
     source          1..9323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1813
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2250..3314
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3383..3577
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3921..4061
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4247..4835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5082..5870)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_feature    6398..6422
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        6576..6755
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6793..6848
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             6850..7398
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     terminator      7428..8135
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    8148..8217
                     /label=CIP7 intron with 12 bp-deletion
                     /note="CIP7 intron with 12 bp-deletion"
     promoter        8248..8294
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    8301..8306
                     /note="Recognition site of Bgl II restriction endonuclease"
     CDS             8324..8833
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     terminator      8864..9117
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9186..9210
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"