pJP5603sacBGm vector (V016483)

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LOCUS       62056_13210        5087 bp DNA     circular SYN 07-JAN-2021
DEFINITION  Suicide vector pJP5603sacBGm DNA, complete sequence.
ACCESSION   LC599391
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Zoolkefli FIR.M., Moriguchi K, Cho Y, Kiyokawa K, Yamamoto S, Suzuki
            K.
  TITLE     Isolation and Analysis of Donor Chromosomal Genes Whose Deficiency 
            is Responsible for Accelerating Bacterial and Trans-kingdom 
            Conjugations by IncP1 T4SS Machinery
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Zoolkefli FIR.M., Moriguchi K, Cho Y, Kiyokawa K, Yamamoto S, Suzuki
            K.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-DEC-2020) Contact:Kazuki Moriguchi Hiroshima 
            University, Biological Science; 1-3-1 Kagamiyama, Higashi-Hiroshima 
            City, Hiroshima 739-8526, Japan
REFERENCE   3  (bases 1 to 5087)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-DEC-2020) Contact:Kazuki Moriguchi Hiroshima University, 
            Biological Science; 1-3-1 Kagamiyama, Higashi-Hiroshima City, 
            Hiroshima 739-8526, Japan"
FEATURES             Location/Qualifiers
     source          1..5087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        338..366
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             555..1085
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     protein_bind    1417..1438
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1453..1483
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1491..1507
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1515..1531
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(1544..1600)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1601..1617)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1689..1757
                     /note="beta-galactosidase; LacZ alpha"
     promoter        2049..2494
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             2495..3913
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     misc_feature    4053..4237
                     /label=TraJ (RP4 mob)
                     /note="TraJ (RP4 mob)"
     oriT            complement(4270..4379)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      4539..4927
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"