pDU1 vector (V016475)

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LOCUS       62056_8645        7971 bp DNA     circular SYN 22-DEC-2019
DEFINITION  Expression vector pDU1, complete sequence.
ACCESSION   MK948095
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Niu T-C., Zhang J-Y., Zhang C-C.
  TITLE     Tight Control of Gene Expression in Anabaena using a hybrid 
            regulatory system consisting of a copper inducible promoter and a 
            theophylline riboswitch
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7971)
  AUTHORS   Niu T-C., Zhang J-Y., Zhang C-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2019) Center for Algal Biology and Applied 
            Research, Institute of Hydrobiology, Donghu Nanlu, Wuhan, Hubei 
            430072, 086
REFERENCE   3  (bases 1 to 7971)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-MAY-2019) Center for Algal Biology and Applied Research, 
            Institute of Hydrobiology, Donghu Nanlu, Wuhan, Hubei 430072, 086"
FEATURES             Location/Qualifiers
     source          1..7971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3801..7971
                     /mol_type="other DNA"
                     /db_xref="taxon:28072"
                     /organism="Nostoc sp. PCC 7524"
     CDS             596..1387
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    1943..2083
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2269..2857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      2952..2976
                     /label=terminator
                     /note="terminator"
                     /regulatory_class="terminator"
     regulatory      2983..3448
                     /note="petE; the promoter region(-475 to -9) of Anabaena
                     petE (id:all0258) gene"
                     /regulatory_class="promoter"
     regulatory      3455..3517
                     /label=theophylline riboswitch
                     /note="theophylline riboswitch"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    3530..3568
                     /label=protein domain linker
                     /note="protein domain linker"
     CDS             3563..3652
                     /label=Twin-Strep-tag
                     /note="two Strep-Tag(R)II moieties connected by a linker 
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
     CDS             3656..3673
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      3740..3787
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     gene            4878..5999
                     /gene="repA"
                     /label=repA
     CDS             4878..5999
                     /codon_start=1
                     /transl_table=11
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="required for plasmid replication in cyanobacteria"
                     /protein_id="QGX42291.1"
                     /translation="MISPESLNTNVSLTLLTRELQTEIILGTYTVRVHTRIGREPCARL
                     WYLCRALDKDGSGHLTLPLPVVQTFLDCSDKSVYRWLQDGKKIGAFRRYKIKAGMITVY
                     LGGMFQVCYNLNLKRWGDVAVVPLVQVLSDLRSLTTGIVTQSFQQKSRYAANRQLKPEY
                     RKLFGAPHPNELVKDTRQSSLKSPEGEVPCVLHISSSRIFVSKSFIHYGTSQKAVSCEL
                     GIHKRTVRRHQKQLGMNRRQLCQAKIEYNQLRHARNNDASEFWAFTGTKTDIGYQVMGD
                     AVVFSDGIAPGAKKRQPNTYQIDATEFDGRLFKVGDKVFMNRCNIYREQFTLTTMSAAR
                     RKYHFKLSQCHFSENRAGRVGNRFVIGCHSGEI"
     CDS             complement(6620..7171)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="required for plasmid replication in cyanobacteria"
                     /protein_id="QGX42292.1"
                     /translation="MKVNGNGRAKILTSDELRRLFSDGFTTPRDRVLFGICLFTGCRVS
                     EALALQTTDIKGETLTFRKSTTKGKLKTRVVDIQPGLAALMADYHPKPGTLFPGMRGVS
                     DRLTRYAADKILRDAAKRIGLEGISTHSFRRTALNQMSSAGIPLRHIQEISGHNDLGTL
                     QRYLEVTPEQRRKAVSVIGF"
     CDS             complement(7543..7896)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="required for plasmid replication in cyanobacteria"
                     /protein_id="QGX42293.1"
                     /translation="MADKTLATFRIDSEEWESFKNLASSESSNASALLTEFVRWYLAGN
                     RFNTPTSHTPTHLDTSLEQRIDNIEQRLDKVTTNNLDNIDEFIDKRIEDNLATRLDKLQ
                     SQLEELRGKSKAR"