Basic Vector Information
- Vector Name:
- pHal7-CvFAPG462V
- Antibiotic Resistance:
- Streptomycin
- Length:
- 6948 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Trisrivirat D, Hughes JMX., Hoeven R, Faulkner M
pHal7-CvFAPG462V vector Map
pHal7-CvFAPG462V vector Sequence
LOCUS 62056_12150 6948 bp DNA circular SYN 14-DEC-2020 DEFINITION Cloning vector pHal7-CvFAPG462V, complete sequence. ACCESSION MW076827 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6948) AUTHORS Trisrivirat D, Hughes JMX., Hoeven R, Faulkner M, Toogood H, Chaiyen P, Scrutton NS. TITLE Promoter engineering for microbial bio-alkane gas production JOURNAL Synth Biol (Oxf) 5 (1), ysaa022 (2020) PUBMED 33263086 REFERENCE 2 (bases 1 to 6948) AUTHORS Trisrivirat D, Hughes JMX., Hoeven R, Faulkner M, Toogood HS, Chaiyen P, Scrutton NS. TITLE Direct Submission JOURNAL Submitted (06-OCT-2020) Chemistry, University of Manchester, 131 Princess Street, Manchester, Lancashire M1 7DN, United Kingdom REFERENCE 3 (bases 1 to 6948) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol (Oxf)"; date: "2020"; volume: "5"; issue: "1"; pages: "ysaa022" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (06-OCT-2020) Chemistry, University of Manchester, 131 Princess Street, Manchester, Lancashire M1 7DN, United Kingdom" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..6948 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(494..1324) /label=pRO1600 Rep /note="replication protein for the broad-host-range plasmid pRO1600 from Pseudomonas aeruginosa" rep_origin 1338..1689 /label=pRO1600 oriV /note="broad-host-range origin of replication from Pseudomonas aeruginosa plasmid pRO1600; requires the pRO1600 Rep protein for replication (West et al., 1994)" terminator complement(1762..1848) /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" regulatory 1897..1959 /regulatory_class="promoter" CDS 1960..3744 /codon_start=1 /transl_table=11 /product="CvFAPG462V" /label=CvFAPG462V /protein_id="QPO14559.1" /translation="MASAVEDIRKVLSDSSSPVAGQKYDYILVGGGTAACVLANRLSAD GSKRVLVLEAGPDNTSRDVKIPAAITRLFRSPLDWNLFSELQEQLAERQIYMARGRLLG GSSATNATLYHRGAAGDYDAWGVEGWSSEDVLSWFVQAETNADFGPGAYHGSGGPMRVE NPRYTNKQLHTAFFKAAEEVGLTPNSDFNDWSHDHAGYGTFQVMQDKGTRADMYRQYLK PVLGRRNLQVLTGAAVTKVNIDQAAGKAQALGVEFSTDGPTGERLSAELAPGGEVIMCA GAVHTPFLLKHSGVGPSAELKEFGIPVVSNLAGVGQNLQDQPACLTAAPVKEKYDGIAI SDHIYNEKGQIRKRAIASYLLGGRGGLTSTGCDRGAFVRTAGQALPDLQVRFVPGMALD PDVVSTYVRFAKFQSQGLKWPSGITMQLIACRPQSTGSVGLKSADPFAPPKLSPGYLTD KDGADLATLRKGIHWARDVARSSALSEYLDGELFPGSGVVSDDQIDEYIRRSIHSSNAI TGTCKMGNAGDSSSVVDNQLRVHGVEGLRVVDASVVPKIPGGQTGAPVVMIAERAAALL TGKATIGASAAAPATVAA" terminator 3835..3929 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS 4038..4850 /label=KanR /note="aminoglycoside phosphotransferase" promoter 5304..5406 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 5626..6414 /label=SmR /note="aminoglycoside adenylyltransferase (Murphy, 1985)" oriT 6595..6703 /label=oriT /note="incP origin of transfer" rep_origin 6797..6948 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
This page is informational only.