pT7-VP3(02V0002G3) vector (V016442)

LOCUS       62056_20850        5001 bp DNA     circular SYN 15-DEC-2020
DEFINITION  Cloning vector pT7-VP3(02V0002G3), complete sequence.
ACCESSION   MN689782
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Potential of avian and mammalian species A rotaviruses to reassort 
            as explored by plasmid only-based reverse genetics
  JOURNAL   Virus Res 286, 198027 (2020)
  PUBMED    32442596
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2019) Unit Viruses in Food, German Federal 
            Institute for Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, 
            Germany
REFERENCE   3  (bases 1 to 5001)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res 
            286, 198027 (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2019) Unit Viruses in Food, German Federal Institute for 
            Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, Germany"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        233..251
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           250..298
     gene            299..2788
                     /gene="VP3"
                     /label=VP3
     CDS             299..2788
                     /codon_start=1
                     /transl_table=11
                     /gene="VP3"
                     /product="VP3"
                     /label=VP3
                     /note="derived from Rotavirus A strain 02V0002G3"
                     /protein_id="QPP12451.1"
                     /translation="MKVIALRRDLVSSYADTQIYKHDESKDYYENAYLISNLTSHNVLY
                     INYYIQVLEILNKSGVASIIATNSEELNLLIKSNYTYDYYRNILYLHDYSYYTLNELRT
                     DQFWLTTTNINDYLPPGWKLTYVGYLGIKTRGHYTHSFTCQNTATDDDIIYDYIYSDET
                     DFLPFLLQALNKRLTSALNYHRLSNRVLRELLYLSVPKNTINIGPRNESMFTLLKYPNI
                     TNYPANEMYVSDLIKLPQEKWVGAENTQFDIGQFKNMCNVLSTIYTYYNKYHDYPKIYM
                     VGSAPSYWIKDLLEIHKFTFETWDPLDTPYSTNHHFEMFSLTDVDKLTDNSILYIDIRT
                     DRNGMDWKQWRTKVEEETKINLDMMYRYLSKGQNRICCCKITAMDIELPISATLLHFPT
                     TKIRSECYILCTKESLLDKKRFVPKGSFYAFINNNKTDNVFFGSEYRLKPTNKFVVALY
                     SLSNENNDRDKMLSFLKKQKNGVITLRVNNTFNYAYRLQFKQTYDYLYLPTDIMRDDTI
                     VTSYDGYAAMHNLSCSLEGKATGNNHLFVSFSDKNYYNIDLYCNHMGISRRSHSIRFSE
                     AATTLSGYMFRDLTNGKFSLINTNVENVVSGHVYNALIYFRYNYRFDLLRWINLHAMNK
                     VSILGGKYYEHAPPELLYACQSALTFAKLQNDLTLIEYVKSVQDFIHDKYKITYADDPN
                     YYISIEFVDLPYKSEVKRPHLTGGLLLINDTEIPTVIEILQTVKEDILMLELTTQYTYM
                     LTDSHYVANVNGYLQFYYKLYMMFYRKNITFGQSRMYIPHITLAKVKKNAIRIDSSRII
                     VKSIFLSQIGTDVEYRI"
     3'UTR           2789..2832
     terminator      2993..3040
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(3209..3225)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      3273..3304
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        3305..3407
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3408..4265
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      4340..4367
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(4379..4966)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"