pYFP-lacY vector (V016441)

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LOCUS       V016441                 6241 bp    DNA     circular SYN 30-MAR-2020
DEFINITION  Exported.
ACCESSION   V016441
VERSION     V016441
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Sarkar S, Tack D, Ross D.
  TITLE     Mutual Information Landscapes as a Performance Metric for
            Biochemical Reaction Networks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Tack D.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2020) Cellular Engineering, National Institute of
            Standards and Technology, 100 Bureau Dr MS8313, Gaithersburg, MD
            20878, USA
REFERENCE   3  (bases 1 to 6241)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JAN-2020) Cellular Engineering, National Institute of Standards
            and Technology, 100 Bureau Dr MS8313, Gaithersburg, MD 20878, USA"
FEATURES             Location/Qualifiers
     source          1..6241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..53
                     /label="L3S3P21"
                     /note="L3S3P21"
                     /regulatory_class="terminator"
     promoter        75..103
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    complement(109..128)
                     /label="lac operator (symmetric)"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The
                     symmetric lac operator was optimized for tight binding of
                     lac repressor."
     misc_RNA        130..180
                     /label="sTRSV HHRz"
                     /note="hammerhead ribozyme from the tobacco ringspot virus
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      212..223
                     /regulatory_class="ribosome_binding_site"
     CDS             230..946
                     /label="EYFP"
                     /note="enhanced YFP"
     regulatory      988..994
                     /regulatory_class="ribosome_binding_site"
     CDS             1001..2251
                     /gene="lacY"
                     /label="Lactose permease"
                     /note="Lactose permease from Escherichia coli (strain K12).
                     Accession#: P02920"
     regulatory      2255..2315
                     /label="L3S2P21"
                     /note="L3S2P21"
                     /regulatory_class="terminator"
     regulatory      2320..2373
                     /label="PLacI"
                     /note="PLacI"
                     /regulatory_class="promoter"
     CDS             2402..3481
                     /label="lacI"
                     /note="lac repressor"
     CDS             3503..4123
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     regulatory      4130..4246
                     /label="araC terminator"
                     /note="araC terminator"
                     /regulatory_class="terminator"
     rep_origin      complement(4693..5237)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(5329..6141)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"