pNRVL- vector (V016427)

LOCUS       62056_18200        3295 bp DNA     circular SYN 03-MAR-2020
DEFINITION  Cloning vector pNRVL', complete sequence.
ACCESSION   MN956532
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Nemeth T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2020) Department of Microbiology, University of 
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (16-JAN-2020) Department of Microbiology, University of Szeged, 
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    239..244
                     /label=NheI site
                     /note="NheI site"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    505..510
                     /label=NheI site
                     /note="NheI site"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    570..575
                     /label=StuI site for cassette release
                     /note="StuI site for cassette release"
     misc_structure  576..1039
                     /note="Site of recombination; CpNEUT5LUpup from C.
                     parapsilosis"
     misc_feature    1040..1047
                     /label=NotI site for selection marker ligation
                     /note="NotI site for selection marker ligation"
     misc_feature    1054..1059
                     /note="BssHII site for selection marker and insert
                     ligation"
     misc_feature    1067..1072
                     /label=XhoI site for insert ligation
                     /note="XhoI site for insert ligation"
     misc_structure  1073..1523
                     /note="Site of recombination; CpNEUT5LDowndown from C. 
                     parapsilosis"
     promoter        complement(1537..1555)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1560..1576)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1590..1688
                     /label=BBa_P1007 promoter
                     /note="BBa_P1007 promoter"
                     /regulatory_class="promoter"
     CDS             1689..2495
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2645..3233
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"