(223)_P_EF1a-mCitrine-T vector (V016420)

LOCUS       62056_46        8543 bp DNA     circular SYN 17-NOV-2020
DEFINITION  Cloning vector (223)_P_EF1a-mCitrine-T, complete sequence.
ACCESSION   MT891333
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Frei T, Cella F, Tedeschi F, Gutierrez J, Stan GB, Khammash M, 
            Siciliano V.
  TITLE     Characterization and mitigation of gene expression burden in 
            mammalian cells
  JOURNAL   Nat Commun 11 (1), 4641 (2020)
  PUBMED    32934213
REFERENCE   2  (bases 1 to 8543)
  AUTHORS   Frei T, Cella F, Tedeschi F, Gutierrez J, Stan G-B., Khammash M, 
            Siciliano V.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2020) Synthetic and Systems Biology for 
            Biomedicine, Italian Institute of Technology-IIT, Largo Barsanti e 
            Martteucci 53, Naples, Campania 80125, Italy
REFERENCE   3  (bases 1 to 8543)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "4641"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-AUG-2020) Synthetic and Systems Biology for Biomedicine, Italian
            Institute of Technology-IIT, Largo Barsanti e Martteucci 53, Naples,
            Campania 80125, Italy"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..266
                     /note="misc_signal; INS"
                     /regulatory_class="other"
     polyA_signal    283..490
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        526..1707
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             1729..2445
                     /label=mEYFP
                     /note="enhanced YFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
     misc_binding    2459..2480
                     /label=miR-FF4 binding site
                     /bound_moiety="miR-FF4"
                     /note="mir FF4 target"
     misc_binding    2481..2502
                     /label=miR-FF4 binding site
                     /bound_moiety="miR-FF4"
                     /note="mir FF4 target"
     misc_binding    2503..2524
                     /label=miR-FF4 binding site
                     /bound_moiety="miR-FF4"
                     /note="mir FF4 target"
     regulatory      complement(2561..2579)
                     /label=SP6 promoter
                     /note="SP6 promoter"
                     /regulatory_class="promoter"
     polyA_signal    2740..2861
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      3000..3265
                     /note="misc_signal; INS"
                     /regulatory_class="other"
     polyA_signal    3282..3506
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        3527..4709
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     misc_feature    4718..4727
                     /label=Kozak sequence
                     /note="Kozak sequence"
     ncRNA           5491..5619
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the
                     human miR-30a microRNA (Zeng et al., 2002)"
     misc_binding    5828..5849
                     /label=miR-FF4 binding site
                     /bound_moiety="miR-FF4"
                     /note="mir FF4 target"
     misc_binding    5850..5871
                     /label=miR-FF4 binding site
                     /bound_moiety="miR-FF4"
                     /note="mir FF4 target"
     misc_binding    5872..5893
                     /label=miR-FF4 binding site
                     /bound_moiety="miR-FF4"
                     /note="mir FF4 target"
     promoter        complement(5931..5949)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      6110..6231
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /regulatory_class="polyA_signal_sequence"
     regulatory      6321..6610
                     /note="misc_signal; INS"
                     /regulatory_class="other"
     rep_origin      complement(6698..7286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7460..8317)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8318..8422)
                     /label=AmpR promoter