pRGC vector (V016418)

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LOCUS       62056_18830        6376 bp DNA     circular SYN 28-FEB-2021
DEFINITION  Cloning vector pRGC, complete sequence.
ACCESSION   MW148406
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Mellini M, Lucidi M, Imperi F, Visca P, Leoni L, Rampioni G.
  TITLE     Generation of genetic tools for gauging multiple gene expression at 
            single cell level
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Mellini M, Lucidi M, Imperi F, Visca P, Leoni L, Rampioni G.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2020) Science, University Roma Tre, Viale 
            Guglielmo Marconi 446, Roma 00146, Italia
REFERENCE   3  (bases 1 to 6376)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-OCT-2020) Science, University Roma Tre, Viale Guglielmo Marconi 
            446, Roma 00146, Italia"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(144..974)
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      988..1339
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             complement(1745..2275)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(2464..2492)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        complement(2510..2614)
                     /label=AmpR promoter
     primer_bind     2853..2869
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2879..2897
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2915..3631)
                     /label=ECFP
                     /note="enhanced CFP"
     regulatory      complement(3638..3649)
                     /label=strong bacterial ribosome binding site
                     /note="strong bacterial ribosome binding site"
                     /regulatory_class="ribosome_binding_site"
     primer_bind     3667..3683
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      3704..3715
                     /label=strong bacterial ribosome binding site
                     /note="strong bacterial ribosome binding site"
                     /regulatory_class="ribosome_binding_site"
     CDS             3722..4435
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     terminator      4458..4552
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(4603..4619)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      4642..4653
                     /label=strong bacterial ribosome binding site
                     /note="strong bacterial ribosome binding site"
                     /regulatory_class="ribosome_binding_site"
     CDS             4660..5367
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     promoter        complement(5390..5408)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(5600..6188)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"