pRiBIN2 vector (V016416)

LOCUS       62056_18855        7852 bp DNA     circular SYN 12-OCT-2021
DEFINITION  Binary vector pRiBIN2 DNA, complete sequence.
ACCESSION   LC652750
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Banno H.
  TITLE     A binary vector for transient expression in plant cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7852)
  AUTHORS   Banno H.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2021) Contact:Hiroharu Banno Chubu University, 
            College of Bioscience and Biotechnology; 1200 Matsumoto-cho, 
            Kasugai, Aichi 487-8501, Japan URL 
            :https://www3.chubu.ac.jp/biology/
REFERENCE   3  (bases 1 to 7852)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-OCT-2021) Contact:Hiroharu Banno Chubu University, College of 
            Bioscience and Biotechnology; 1200 Matsumoto-cho, Kasugai, Aichi 
            487-8501, Japan URL :https://www3.chubu.ac.jp/biology/"
FEATURES             Location/Qualifiers
     source          1..7852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      56..644
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1116..1137
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1152..1182
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1190..1206
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1214..1230
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1245..5434
                     /label=Ri plasmid replication origin
                     /note="Ri plasmid replication origin"
     misc_feature    complement(5912..5936)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    5947..5984
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     misc_feature    complement(6049..6073)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(6476..6492)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(6714..7526)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"