Basic Vector Information
- Vector Name:
- pTonB2-kanamycin-resistance-protein-gene
- Antibiotic Resistance:
- Kanamycin
- Length:
- 7318 bp
- Type:
- Cloning vector
- Replication origin:
- pBBR1 oriV
- Source/Author:
- Grogan CL, Lampe DJ.
pTonB2-kanamycin-resistance-protein-gene vector Map
pTonB2-kanamycin-resistance-protein-gene vector Sequence
LOCUS V016415 7318 bp DNA circular SYN 24-FEB-2021 DEFINITION Exported. ACCESSION V016415 VERSION V016415 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 7318) AUTHORS Grogan CL, Lampe DJ. TITLE Novel Asaia sp. type II secretion signals for Plasmodium inhibition in Anopheles stephensi JOURNAL Unpublished REFERENCE 2 (bases 1 to 7318) AUTHORS Grogan CL, Lampe DJ. TITLE Direct Submission JOURNAL Submitted (18-OCT-2020) Biological Sciences, Duquesne University, 600 Forbes Ave, Pittsburgh, PA 15219-3016, USA REFERENCE 3 (bases 1 to 7318) AUTHORS . TITLE Direct Submission COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (18-OCT-2020) Biological Sciences, Duquesne University, 600 Forbes Ave, Pittsburgh, PA 15219-3016, USA" FEATURES Location/Qualifiers source 1..7318 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 458..1249 /label="NeoR/KanR" /note="aminoglycoside phosphotransferase" regulatory 1552..1763 /label="PnptII" /note="PnptII" /regulatory_class="promoter" misc_feature 1767..1916 /label="TonB dependent receptor 2 secretion signal" /note="TonB dependent receptor 2 secretion signal" CDS 1923..3272 /gene="phoA" /label="Alkaline phosphatase" /note="Alkaline phosphatase from Escherichia coli (strain K12). Accession#: P00634" CDS complement(4867..5526) /label="pBBR1 Rep" /note="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" rep_origin complement(5527..6296) /direction=LEFT /label="pBBR1 oriV" /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication"
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