AGG1221-pCHIKVLuc vector (V016413)

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LOCUS       62056_301        6610 bp DNA     circular SYN 12-AUG-2020
DEFINITION  Cloning vector AGG1221:pCHIKVLuc, complete sequence.
ACCESSION   MT119958
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Tng PYL., Carabajal Paladino L, Verkuijl SAN., Purcell J, Merits A, 
            Leftwich PT, Fragkoudis R, Noad R, Alphey L.
  TITLE     Cas13b-dependent and Cas13b-independent RNA knockdown of viral 
            sequences in mosquito cells following guide RNA expression
  JOURNAL   Commun Biol 3 (1), 413 (2020)
  PUBMED    32737398
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Tng PYL., Carabajal Paladino L, Verkuijl S, Purcell J, Merits A, 
            Leftwich PT, Fragkoudis R, Noad R, Alphey L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2020) Arthropod Genetics Group, The Pirbright 
            Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK
REFERENCE   3  (bases 1 to 6610)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol"; date: "2020"; volume: "3"; issue: "1"; pages: "413"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-FEB-2020) Arthropod Genetics Group, The Pirbright Institute, Ash
            Road, Pirbright, Woking, Surrey GU24 0NF, UK"
FEATURES             Location/Qualifiers
     source          1..6610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        47..529
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     intron          1273..1342
                     /note="adh"
     misc_feature    1345..1353
                     /label=Kozak sequence
                     /note="Kozak sequence"
     misc_feature    1354..1626
                     /gene="CHIKV-Ubi-luciferase polyprotein"
                     /label=CHIKV nsP2
                     /note="CHIKV nsP2"
     CDS             1627..1851
                     /label=ubiquitin-K48R
                     /note="human ubiquitin variant in which Lys-48 has been
                     mutated to arginine"
     CDS             1852..3501
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(3545..3666)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4085..4673)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4847..5704)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5705..5809)
                     /label=AmpR promoter
     rep_origin      5836..6291
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    6422..6470
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6484..6575
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"