Basic Vector Information
- Vector Name:
- AGG1221-pCHIKVLuc
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6610 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Tng PYL., Carabajal Paladino L, Verkuijl SAN., Purcell J
- Promoter:
- IE1
AGG1221-pCHIKVLuc vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
AGG1221-pCHIKVLuc vector Sequence
LOCUS 62056_301 6610 bp DNA circular SYN 12-AUG-2020
DEFINITION Cloning vector AGG1221:pCHIKVLuc, complete sequence.
ACCESSION MT119958
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6610)
AUTHORS Tng PYL., Carabajal Paladino L, Verkuijl SAN., Purcell J, Merits A,
Leftwich PT, Fragkoudis R, Noad R, Alphey L.
TITLE Cas13b-dependent and Cas13b-independent RNA knockdown of viral
sequences in mosquito cells following guide RNA expression
JOURNAL Commun Biol 3 (1), 413 (2020)
PUBMED 32737398
REFERENCE 2 (bases 1 to 6610)
AUTHORS Tng PYL., Carabajal Paladino L, Verkuijl S, Purcell J, Merits A,
Leftwich PT, Fragkoudis R, Noad R, Alphey L.
TITLE Direct Submission
JOURNAL Submitted (26-FEB-2020) Arthropod Genetics Group, The Pirbright
Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK
REFERENCE 3 (bases 1 to 6610)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Commun
Biol"; date: "2020"; volume: "3"; issue: "1"; pages: "413"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(26-FEB-2020) Arthropod Genetics Group, The Pirbright Institute, Ash
Road, Pirbright, Woking, Surrey GU24 0NF, UK"
FEATURES Location/Qualifiers
source 1..6610
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 47..529
/label=hr5 enhancer
/note="baculovirus early transcription enhancer"
intron 1273..1342
/note="adh"
misc_feature 1345..1353
/label=Kozak sequence
/note="Kozak sequence"
misc_feature 1354..1626
/gene="CHIKV-Ubi-luciferase polyprotein"
/label=CHIKV nsP2
/note="CHIKV nsP2"
CDS 1627..1851
/label=ubiquitin-K48R
/note="human ubiquitin variant in which Lys-48 has been
mutated to arginine"
CDS 1852..3501
/label=luciferase
/note="firefly luciferase"
polyA_signal complement(3545..3666)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4085..4673)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4847..5704)
/label=AmpR
/note="beta-lactamase"
promoter complement(5705..5809)
/label=AmpR promoter
rep_origin 5836..6291
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal 6422..6470
/label=poly(A) signal
/note="synthetic polyadenylation signal"
misc_feature 6484..6575
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
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