pJV298-Tyr1 vector (V016407)

LOCUS       62056_13245        5295 bp DNA     circular SYN 22-JUL-2020
DEFINITION  Cloning vector pJV298-Tyr1, complete sequence.
ACCESSION   MT346028
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Lee MS, Hung CS, Phillips DA, Buck CC, Gupta MK, Lux MW.
  TITLE     Silk fibroin as an additive for cell-free protein synthesis
  JOURNAL   Synth Syst Biotechnol 5 (3), 145-154 (2020)
  PUBMED    32637668
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Lee MS.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2020) US Army CCDC Chemical Biological Center, 
            8567 Ricketts Point Road, Gunpowder, MD 21010, USA
REFERENCE   3  (bases 1 to 5295)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Syst 
            Biotechnol"; date: "2020"; volume: "5"; issue: "3"; pages: "145-154"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-APR-2020) US Army CCDC Chemical Biological Center, 8567 Ricketts
            Point Road, Gunpowder, MD 21010, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..546)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(770..1849)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(1850..1927)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        2161..2189
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    2197..2213
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     gene            2277..3170
                     /gene="tyr1"
                     /label=tyr1
     CDS             2277..3170
                     /codon_start=1
                     /transl_table=11
                     /gene="tyr1"
                     /product="tyrosinase"
                     /label=tyr1
                     /note="Tyr1 tyrosinase; derived from Bacillus megaterium"
                     /protein_id="QLI61466.1"
                     /translation="MGNKYRVRKNVLHLTDTEKRDFVRTVLILKEKGIYDRYIAWHGAA
                     GKFHTPPGSDRNAAHMSSAFLPWHREYLLRFERDLQSINPEVTLPYWEWETDAQMQDPS
                     QSQIWSADFMGGNGNPIKDFIVDTGPFAAGRWTTIDEQGNPSGGLKRNFGATKEAPTLP
                     TRDDVLNALKITQYDTPPWDMTSQNSFRNQLEGFINGPQLHNRVHRWVGGQMGVVPTAP
                     NDPVFFLHHANVDRIWAVWQIIHRNQNYQPMKNGPFGQNFRDPMYPWNTTPEDVMNHRK
                     LGYVYDIELRKSKRSS"
     oriT            3587..3696
                     /label=oriT
                     /note="incP origin of transfer"
     gene            complement(4103..4741)
                     /gene="cat"
                     /label=cat
     CDS             complement(4103..4741)
                     /codon_start=1
                     /transl_table=11
                     /gene="cat"
                     /product="Cat"
                     /label=cat
                     /note="chloramphenicol acetyltransferase"
                     /protein_id="QLI61467.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRCLMNTTVLR"
     promoter        complement(4742..4844)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"