pRY-23E5-iLOV vector (V016398)

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LOCUS       V016398                 6575 bp    DNA     circular SYN 13-MAY-2019
DEFINITION  Exported.
ACCESSION   V016398
VERSION     V016398
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Suman J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2018) Dpt. of Biochemistry and Microbiology,
            University of Chemistry and Technology Prague, Technicka 3, Prague,
            Czech Republic 16628, Czech Republic
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (24-OCT-2018) Dpt. of Biochemistry and Microbiology, University of
            Chemistry and Technology Prague, Technicka 3, Prague, Czech Republic
            16628, Czech Republic"
FEATURES             Location/Qualifiers
     source          1..6575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      650..747
                     /label="23E5 promoter region from C. jejuni"
                     /note="23E5 promoter region from C. jejuni"
                     /regulatory_class="promoter"
     CDS             751..1068
                     /label="miniSOG"
                     /note="mini singlet oxygen generator, a fluorescent
                     flavoprotein suitable for correlated light and electron
                     microscopy (Shu et al., 2011)"
     CDS             complement(1439..1807)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(1840..1949)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             2054..2410
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /label="AmpR"
                     /note="class A beta-lactamase; ampicilin resistance
                     protein"
                     /protein_id="QCO69682.1"
                     /translation="MTPKQGYAAEKIRRAMATTLRKLLTGELLTLASRQQLIDWMEADK
                     VAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERN
                     RQIAEIGASLIKHW"
     rep_origin      2581..3169
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             3618..4238
                     /note="Chloramphenicol acetyltransferase from Campylobacter
                     coli. Accession#: P22782"
                     /label="Chloramphenicol acetyltransferase"
     CDS             complement(5004..6059)
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /label="RepB family plasmid replication initiator protein"
                     /protein_id="QCO69684.1"
                     /translation="MQMSEIVKYHNDFNKIQLPSFTEQEQNLLFLIFARIKEKGIDNVI
                     NLYANDFKINEKLSNSREYLTENINSLKYKFFKANFKQIIETKTEVIHKYVNLFETMEI
                     HYVKRNPDDGYDESTLFNRITLKINPDFAYLVNQLTTNFTAFELEEFIALSGKYAKTLY
                     RLLKQFRTTGKAYFEWDEFCKVMDIPQDYRQSEVDKWILKPAIKELSKERNLFDQIRVP
                     FKNLAYEKEKTAGRGRGGKVSGISFTFKPENIEMQKLENESQKIMSDEQKYLKILNNMK
                     LNQARFNYNDKLWQFNDFDFNEFKIIAVELAVELVRDEYENLNFGNHMHFNAKNQEQFF
                     KMIDTFRNGIR"