pTS9 vector (V016393)

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LOCUS       62056_21595        5191 bp DNA     circular SYN 09-MAY-2021
DEFINITION  Cloning vector pTS9, complete sequence.
ACCESSION   MW835298
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Schnabel T.
  TITLE     Engineering post-translational regulation of glutamine synthetase 
            for controllable ammonia production in the plant-symbiont A. 
            brasilense
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Schnabel T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2021) Bioengineering, Stanford University, 443 Via
            Ortega, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 5191)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-MAR-2021) Bioengineering, Stanford University, 443 Via Ortega, 
            Stanford, CA 94305, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      139..727
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      778..812
                     /label=pJ23101
                     /note="pJ23101"
                     /regulatory_class="promoter"
     regulatory      828..842
                     /label=B0030
                     /note="B0030"
                     /regulatory_class="ribosome_binding_site"
     gene            849..1868
                     /gene="pheS*"
                     /label=pheS*
     CDS             849..1868
                     /codon_start=1
                     /transl_table=11
                     /gene="pheS*"
                     /product="phenylalanine--tRNA ligase alpha subunit"
                     /function="toxic in presence of 4-chlorophenylalanine"
                     /label=pheS*
                     /protein_id="QUQ60671.1"
                     /translation="MLDALKDELLSQVNAAGDLAALEEVRVTALGKKGRITGFMKELGG
                     LSPDERRERGQQLNALKDEIAAAIDGRKADLARAHLEARLQAERIDVTLPVRPETEGRI
                     HPISQTIDEMVAIFAEMGFSVAEGPDVEDDFHNFTALNFPPGHPARDMHDTFYLPDAGD
                     KKMLLRTHTSPVQVRTMLNKKPPIRIIAPGRTYRSDYDMTHTPMFHQIEGLVIDEATHM
                     GHLKGCLIEFCRAFFDVDDLPLRFRPSFFPFAEPSAEVDIGCSRKGGELKLGNYGDWLE
                     ILGCGMVHPNVLEACGIDSTKYQGFGFGMGIERVAMLKYGIPDLRTFFEADLRWLKHY"
     regulatory      1885..1997
                     /label=pKan
                     /note="pKan"
                     /regulatory_class="promoter"
     oriT            complement(1885..1936)
                     /direction=LEFT
                     /note="oriT"
     CDS             2004..2795
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    2830..2873
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             2905..2916
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     misc_feature    3299..3309
                     /label=threeway stop
                     /note="threeway stop"
     misc_feature    3316..3362
                     /note="insert spacer for knock-out or circuit of interest
                     for knock-in"
     misc_feature    3369..3379
                     /label=threeway stop
                     /note="threeway stop"
     misc_feature    3380..3800
                     /note="downstream overlap to chromosome (here an example 
                     fragment of A. brasilense Sp245)"
     misc_feature    3801..3821
                     /label=multiple cloning site
                     /note="multiple cloning site"
     oriT            complement(3913..4022)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        4194..4298
                     /label=AmpR promoter
     CDS             4299..5156
                     /label=AmpR
                     /note="beta-lactamase"