pcDNA3.1Zeo(-)-HBV1.1--E2 vector (V016388)

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LOCUS       V016388                 8237 bp    DNA     circular SYN 21-DEC-2019
DEFINITION  Exported.
ACCESSION   V016388
VERSION     V016388
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Wang W, Ni Y, Urban S.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2019) Molecular Virology, Department of
            Infectiology, Heidelberg University Hospital, Inf344, Heidelberg
            69120, Deutschland
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (03-NOV-2019) Molecular Virology, Department of Infectiology,
            Heidelberg University Hospital, Inf344, Heidelberg 69120,
            Deutschland"
FEATURES             Location/Qualifiers
     source          1..8237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1324..3849
                     /gene="P"
                     /label="Protein P"
                     /note="Protein P from Hepatitis B virus genotype E (isolate
                     Cote d'Ivoire/ABI-129/2003). Accession#: Q80IU7"
     polyA_signal    4250..4474
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4520..4948
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4962..5292
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        5340..5387
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5406..5777
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    5910..6043
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6080..6096)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6104..6120)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6128..6158)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6173..6194)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6482..7070)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7244..8101)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8102..8206)
                     /label="AmpR promoter"