pTetON-BMP2-7 vector (V016369)

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LOCUS       V016369                 7305 bp    DNA     circular SYN 01-MAY-2019
DEFINITION  Exported.
ACCESSION   V016369
VERSION     V016369
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Feichtinger GA.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2019) Division of Oral Biology, University of
            Leeds, Wellcome Trust Brenner Building, Level 7, St. James
            University Hospital Campus, Leeds, West Yorkshire LS9 7TF, United
            Kingdom
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (22-JAN-2019) Division of Oral Biology, University of Leeds,
            Wellcome Trust Brenner Building, Level 7, St. James University
            Hospital Campus, Leeds, West Yorkshire LS9 7TF, United Kingdom"
FEATURES             Location/Qualifiers
     source          1..7305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    12..30
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_binding    complement(48..66)
                     /label="tet binding site"
                     /bound_moiety="tet"
                     /note="tet operator"
     misc_binding    84..102
                     /label="tet binding site"
                     /bound_moiety="tet"
                     /note="tet operator"
     misc_binding    119..137
                     /label="tet binding site"
                     /bound_moiety="tet"
                     /note="tet operator"
     misc_binding    155..173
                     /label="tet binding site"
                     /bound_moiety="tet"
                     /note="tet operator"
     promoter        185..223
                     /label="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             324..1511
                     /gene="BMP2"
                     /label="Bone morphogenetic protein 2"
                     /note="Bone morphogenetic protein 2 from Homo sapiens.
                     Accession#: P12643"
     polyA_signal    1691..1772
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     polyA_signal    2232..2366
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(2379..3122)
                     /label="rtTA-Advanced"
                     /note="improved tetracycline-controlled transactivator"
     promoter        complement(3225..3428)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(3429..3808)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3889..4477)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4651..5508)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5509..5613)
                     /label="AmpR promoter"
     polyA_signal    complement(5646..5727)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(5863..7155)
                     /gene="BMP7"
                     /label="Bone morphogenetic protein 7"
                     /note="Bone morphogenetic protein 7 from Homo sapiens.
                     Accession#: P18075"
     regulatory      complement(7184..7253)
                     /label="minimal CMV promoter 2"
                     /note="minimal CMV promoter 2"
                     /regulatory_class="promoter"
     misc_binding    complement(7265..7283)
                     /label="tet binding site"
                     /bound_moiety="tet"
                     /note="tet operator"