pMMZ284 vector (V016367)

LOCUS       62056_17185        6172 bp DNA     circular SYN 21-APR-2021
DEFINITION  Cloning vector pMMZ284, complete sequence.
ACCESSION   MW731449
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Mansouri M, Hussherr M-D., Strittmatter T, Buchmann P, Xue S, 
            Camenisch G, Fussenegger M.
  TITLE     Smart-Watch-Programmed Green-Light-Operated Percutaneous Control of 
            Therapeutic Transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6172)
  AUTHORS   Mansouri M, Hussherr M-D., Strittmatter T, Buchmann P, Xue S, 
            Camenisch G, Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2021) DBSSE, ETH, Mattenstrasse 26, Basel, Choose 
            A State 4058, Switzerland
REFERENCE   3  (bases 1 to 6172)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAR-2021) DBSSE, ETH, Mattenstrasse 26, Basel, Choose A State 
            4058, Switzerland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(49..65)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(73..89)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(97..127)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(142..163)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(451..1036)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1210..2067)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2068..2172)
                     /label=AmpR promoter
     promoter        2494..2512
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2544..3938
                     /codon_start=1
                     /transl_table=11
                     /product="Myr-TtCBD-nGFP"
                     /label=Myr-TtCBD-nGFP
                     /note="Supercharged membrane anchored TtCBD"
                     /protein_id="QTW97888.1"
                     /translation="MGCINSKRKDASPEDLGTGLLEALLRGDLAGAEALFRRGLRFWGP
                     EGILEHLLLPVLREVGEAWHRGEIGVAEEHLASTFLRARLQELLDLAGFPPGPPVLVTT
                     PPGERHEIGAMLAAYHLRRKGVPALYLGPDTPLPDLRALARRLGAGAVVLSALLSEPLR
                     ALPDGALKDLAPRVFLGGQGAGPEEARRLGAEYMEDLKGLAEALWLPRGPEKEAIASKG
                     EELFDGVVPILVELDGDVNGHEFSVRGEGEGDATEGELTLKFICTTGELPVPWPTLVTT
                     LTYGVQCFSDYPDHMDQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNR
                     IELKGIDFKEDGNILGHKLEYNFNSHDVYITADKQENGIKAEFEIRHNVEDGSVQLADH
                     YQQNTPIGDGPVLLPDDHYLSTESALSKDPNEDRDHMVLLEFVTAAGIDHGMDELYKTA
                     SGSTGV"
     polyA_signal    3975..4199
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4245..4673
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4687..5016
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5083..5874
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    6051..6172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"